Hematology |
Coagulation |
Urinalysis |
Clinical Chemistry
Special Chemistry |
Immunoassays
Sample Collection
500 uL EDTA Whole blood + slide made at site. Do not freeze!
Complete Blood Count
Red Cell Count
Hemoglobin
Hematocrit
Mean Corpuscular Volume (MCV)
Mean Corpuscular Hemoglobin (MCH)
Mean Corpuscular Hemoglobin Concentration (MCHC)
Platelet Count
Leukocyte Count
Leukocyte Differential
RBC Morphology
Reticulocyte Count
Bone Marrow Evaluation
Myeliod/Erythroid Ratio and cytology with interpretive report
TOP
500uL of whole blood or special stained cells, 2 slides
2 slides of bone marrow painted on slides, unstained; hematology data
Prothrombin Time (PT)
Activated Partial Prothrombin Time (APTT)
Fibrinogen
Fibrin Degredation Products (FDP)
D-Dimer
500 uL frozen citrate plasma (1)
500 uL frozen citrate plasma (1)
500 uL frozen citrate plasma (1)
500 uL frozen citrate plasma (1)
500 uL frozen citrate plasma (1)
(1) Sample must be collected in tube with ratio of 1 part sodium
Citrate and 9 parts blood. Special tubes must be made for small volumes.
TOP
2 mL or entire specimen of urine refrigerated unspun. Provide total volume. Preferred collected over a timed period. Provide time period.
Appearance |
Ketones* |
*Assays performed as semiquantitative dipstick method
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Minimum sample for one test 150 uL. Up to 20 tests can be performed on 350 uL of frozen serum. Separate from cells within I hour of collection.
Alkaline Phosphatase (ALP) |
Glucose |
Clinical Chemistry tests can be performed on urine samples as quantitative
assays. Indirect Bilirubin is a calculated parameter and requires Total and Direct Bilirubin.
(No additional sample volume.)
Globulin and A/G ratio are calculated parameters and require Total Protein and Albumin. (
No additional sample volume.)
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Alkaline Phosphatase Isoenzymes
Angiotensin Converting Enzyme (ACE)
Apolipoprotein
Bile Acids
CK Isoenzymes
Cholinesterase
CH50 Total Complement
C3 Complement
C4 Complement
Ferritin
Free Fatty Acids
Free Hemoglobin
HDL Cholesterol
Haptoglobin
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Iron
LD Isoenzymes
LDL Cholesterol
Lactic Acid
Myoglobin
NAG
5'-Nucleotidase
Phopholipids
Protein Electrophoresis
Sorbitol Dehydrogenase (SDH)
Unsaturated Iron Binding capacity (UIBC)
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500 uL frozen serum
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 15 uL pipetted
500 uL serum preferred, 250 uL minimum
200 uL serum preferred, 100 uL minimum 50 uL pipetted
500 uL of EDTA whole blood
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 20 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL Heparinized plasma preferred, 100 uL minimum
25 uL pipetted. No vaccum tubes, use syringe.
200 uL serum preferred, 100 uL minimum 2 uL pipetted
200 uL serum preferred, 100 uL minimum 50 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 12 uL pipetted
500 uL serum room temperature, ship immediately, do not freeze.
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL Fluoride Plasma,
Whole blood - Prepare 1 part blood and 1 part 8% percholic acid precipitate.
Freeze supernatant.
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL urine unpreserved preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 5 uL pipetted
200 uL serum preferred, 100 uL minimum 25 uL pipetted
500 uL serum
200 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 8 uL pipetted
Adrenocorticotropic Hormone (ACTH)
Aldosterone
Antidiuretic Hormone (ADH)
Calcitonin
Catacholamines
Corticosterone
Cortisol
DHEA
DHEA Sulfate
Dihydrotestosterone
Estradiol
Folic Acid
Follical Stimulating Hormone (FSH)
Glucagon
Growth Hormone
Histamine
Insulin-Like growth Factor (IGF-1)
Insulin
Leutenizing hormone (LH)
Leptin
N-telopeptide
Osteocalcin
Parathyroid Hormone (PTH-intact)
Pro-BNP
Progesterone
Prolactin
Thryroid Stimulating Hormone (TSH)
Testosterone
Thyroxine (T4)
Free T4
Tri-iodothyronine (T3)
T3 Uptake
Troponin I
Vitamin B12
Vitamin D 1,25 OH
TOP
200 uL EDTA plasma preferred, 100 uL minimum 75 uL pipetted. Collect in cold siliconized
tube, separate and freeze immediately.
500 uL serum preferred, 150 uL minimum 100 uL pipetted
750 uL serum preferred, 500 uL minimum 400 uL pipetted
Rat - 500 uL of Heparinized plasma preferred, 250 uL minimum, 200 uL pipetted.
Non-Rodents - 500 uL of Heparinized plasma preferred, 2000 uL minimum, 75 uL
pipetted.
500 uL serum preferred, 250 uL minimum 200 uL pipetted
200 uL serum preferred, 150 uL minimum 50 uL pipetted
200 uL serum preferred, 120 uL minimum 10 uL pipetted
500 uL serum preferred, 150 uL minimum 100 uL pipetted
500 uL serum preferred, 100 uL minimum 25 uL pipetted
200 uL serum preferred, 100 uL minimum 50 uL pipetted
200 uL serum preferred, 100 uL minimum 20 uL pipetted
500 uL serum preferred, 300 uL minimum 250 uL pipetted
500 uL Heparinized Plasma preferred, 250 uL minimum 200 uL pipetted
For Whole Blood - Record Hematocrit. 100 uL of blood in 2 mL of 1% ascorbic
acid (fresh) Freeze immediately.
500 uL serum preferred, 150 uL minimum 100 uL pipetted
500 uL EDTA Plasma with 5000 U Trasylal/10 mL of blood, 250 ul minimum
500 uL serum preferred, 200 uL minimum 100 uL pipetted
250 uL EDTA plasma, 100 uL minimum Freeze immediately.
Rat - 100 uL serum preferred, 50 uL minimum 10 uL pipetted.
NHP - 100 uL serum preferred, 50 uL minimum 20 uL pipetted.
Rat, canine - 100 uL serum preferred EDTA or heparin acceptable, 50 uL minimum
25 uL pipetted;
NHP 250 uL serum preferred, 200 uL minimum 100 uL pipetted
500 uL serum preferred, 150 uL minimum 100 uL pipetted
500 uL serum preferred, 150 uL minimum 100 uL pipetted
Serum - 100 uL preferred, 75 uL minimum 50 uL pipetted
Urine - 500 uL preferred, 50 uL minimum 25 uL pipetted
Rat - 100 uL serum preferred (Heparin acceptable), 50 uL minimum 20 uL pipetted.
NHP - 100 uL serum preferred, 50 uL minimum 10 uL pipetted
Rat - 500 uL serum preferred, 250 uL minimum 200 uL pipetted
Non-Rodent - 200 uL serum preferred, 150 uL minimum 50 uL pipetted
200 uL serum preferred, 150 uL minimum 100 uL pipetted
200 uL serum preferred, 125 uL minimum 25 uL pipetted No gel seperators
500 uL serum preferred, 200 uL minimum 100 uL pipetted
Canine - 100 uL serum preferred, 50 uL minimum 25 uL pipetted
500 uL serum preferred, 150 uL minimum 100 uL pipetted
Canine - 150 uL serum preferred, 125 uL minimum 25 uL pipetted
150 uL serum preferred (Heparin acceptable), 120 uL minimum 20 uL pipetted
125 uL serum preferred (Heparin acceptable), 115 uL minimum 15 uL pipetted
500 uL serum preferred, 200 uL minimum
150 uL serum preferred (Heparin acceptable), 125 uL minimum 25 uL pipetted
500 uL serum preferred, 200 uL minimum
175 uL serum preferred (Heparin acceptable), 150 uL minimum 50 uL pipetted
250 uL serum preferred (Heparin acceptable), 200 uL minimum 200 uL pipetted
500 uL serum preferred (Heparin acceptable), 250 uL minimum 250 uL pipetted
Sample collection is very important in the integrity and eventual interpretation of the data.
There are many pre-analytical variables that can affect the results of clinical pathology testing.
Collecting the correct sample collection tube, collection site, sample storage and shipment can all
affect the result before analysis even begins.
For mice and rats, the typical blood collection procedures require that the animals are anesthetized
and restrained. It is important to use the same collection methods that work best for your facility.
Always use the same collection site as well(1,2). Values can vary from one collection site to another.
For other animals as well as rats and mice, it is important in some cases to collect blood at a certain
time of day depending on the circadian rhythm. Fasting may be important for some tests. Rats and mice
are not truly fasting for almost 24 hours, although debates continue if rats should be fasted.
Please check the list below to determine the correct sample type (serum or plasma) for the test. Some
anticoagulants are not acceptable for certain tests and will invalidate the test results. Plasma can be
obtained from the use of several different anti-coagulants. Each type of anti-coagulant changes the plasma.
Not all anticoagulants are acceptable for all tests.
Sample storage is also important. The test manufacturer has tested samples for the best storage conditions
and the time that samples are stable at these conditions. If these conditions cannot be met, it is important
to send the samples for analysis as soon as possible.
1. Neptun, D, Smith, C and Irons, R, Effects of Sampling Site and Collection Method on Variations in Baseline Clinical Pathology Parameters in Fischer-344 Rats, I Clinical Chemistry, Fundamental and Applied Toxicology, 5, 1180-1185 (1985).
2. Smith, C, Neptun, D and Irons, R, Effects of Sampling Site and Collection Method on Variations in Baseline Clinical Pathology Parameters in Fischer-344 Rats, II Hematology, Fundamental and Applied Toxicology, 7, 658-663 (1986).
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